Enrichment and single-cell analysis of circulating tumor cells

Enrichment and single-cell analysis of circulating tumor cells

Up to 90% of cancer-related deaths are caused by metastatic cancer. Circulating tumor cells (CTCs), a type of cancer cell that spreads through the blood after detaching from a solid tumor, are essential for the establishment of distant metastasis for a given cancer. As a new type of liquid biopsy, analysis of CTCs offers the possibility to avoid invasive tissue biopsy procedures with practical ...

Microfluidic enrichment for the single cell analysis of circulating tumor cells

Resistance to drug therapy is a major concern in cancer treatment. To probe clones resistant to chemotherapy, the current approach is to conduct pooled cell analysis. However, this can yield false negative outcomes, especially when we are analyzing a rare number of circulating tumor cells (CTCs) among an abundance of other cell types. Here, we develop a microfluidic device that is able to perfo...

Single-cell analyses of circulating tumor cells

Circulating tumor cells (CTCs) are a population of tumor cells mediating metastasis, which results in most of the cancer related deaths. The number of CTCs in the peripheral blood of patients is rare, and many platforms have been launched for detection and enrichment of CTCs. Enumeration of CTCs has already been used as a prognosis marker predicting the survival rate of cancer patients. Yet CTC...

AB34. Circulating tumor cell enrichment and analysis in prostate cancer

Botulinum toxin has recently been approved by the United States FDA for idiopathic and neurogenic detrusor overactivity (overactive bladder). This is an easy clinic procedure that can be performed cystoscopically under local anesthesia with very good success. We will discuss the mechanism of action of this drug, the efficacy, and clinical precautions to ensure a safe and successful result for t...

Single Cell Characterization of Prostate Cancer Circulating Tumor Cells